For Research Use Only (RUO). Not for use in diagnostic procedures.

VIASURE HBV q Real Time PCR Detection Reagents RUO is a qPCR test designed for the quantitative detection of HBV DNA in human samples. After DNA isolation, the detection of HBV is performed by the amplification of a conserved region of the S gene, using specific primers and fluorescent-labelled probes.

VIASURE HBV q Real Time PCR Detection Reagents RUO contains in each well all the components necessary for real time PCR assay (specific primers/probes, dNTPs, buffer and polymerase) in a stabilized format, as well as an endogenous internal control to monitor the extraction process and/or discard the inhibition of the polymerase activity. The assay uses a human housekeeping gene as an endogenous internal control (EIC) (human RNase P gene). Human housekeeping genes are involved in basic cell maintenance and, therefore, are expected to be present in all nucleated human cells and maintain relatively constant expression levels.

In addition, this kit contains 5 lyophilized vials (HBV1Q1 to HBV1Q5) with different standardized concentrations of HBV specific DNA (HBV q Quantitative Standards) for the quantification of HBV viral DNA in research samples. These quantitative standards are calibrated against the 4th WHO International Standard for HBV DNA for Nucleic Acid Amplification Techniques (NIBSC code 10/266), ensuring the quality of the results and the comparison with results from other laboratories.