VIASURE Real Time PCR Detection Kits

Description

For Research Use Only (RUO). Not for use in diagnostic procedures.

VIASURE RNase P Real Time PCR Detection Reagents RUO is a non-automatized qPCR test designed for the qualitative detection of DNA from human ribonuclease (RNase) P gene. This target, an endogenous human gene, is of great utility to verify sample integrity and proper nucleic acid (NA) extraction.

DNA from RNase P gene is extracted from human samples, amplified using qPCR and detected using fluorescent reporter dye probes specific for RNase P gene.

This test must be used for research purposes and has no medical objective.

VIASURE RNase P Real Time PCR Detection Reagents RUO is test designed for the qualitative detection of human RNase P gene in human samples. After DNA insolation, the identification of RNase P gene is performed by its amplification, using specific primers and a fluorescent–labelled probes.
VIASURE RNase P Real Time PCR Detection Reagents RUO contains in each well all the components necessary for real time PCR assay (specific primers/probes, dNTPs, buffer and polymerase) in a stabilized format. The amplification of the RNase P gene allows the final user of the kit to verify the sample integrity, the extraction process and/or discard the inhibition of the polymerase activity. Human housekeeping genes are involved in basic cell maintenance and, therefore, are expected to be present in all nucleated human cells and maintain relatively constant expression levels.

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